中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (11): 1653-1658.doi: 10.3969/j.issn.2095-4344.2014.11.003

• 骨组织构建 bone tissue construction • 上一篇    下一篇

自体骨髓浓缩物修复种植体周围的骨缺损

杨 颖1，仲维剑2，刘 国3，马国武2   

  1. 温州医科大学附属口腔医院，1种植科，3牙体牙髓科，浙江省温州市 325027；2大连医科大学口腔医学院口腔颌面外科，辽宁省大连市 116085
  • 修回日期:2014-01-11 出版日期:2014-03-12 发布日期:2014-03-12
  • 通讯作者: 马国武，博士，教授，大连医科大学口腔医学院口腔颌面外科，辽宁省大连市 116085
  • 作者简介:杨颖，男，1987年生，河南省许昌市人，汉族，大连医科大学2013年口腔临床医学硕士，医师，主要从事种植牙周围骨再生、骨重建的研究。

Autologous bone marrow aspirate concentrate repairs peri-implant bone defect

Yang Ying1, Zhong Wei-jian2, Liu Guo3, Ma Guo-wu2   

  1. 1Department of Oral Implantology, Affiliated Stomatological Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China; 2Department of Oral and Maxillofacial Surgery, Stomatology College of Dalian Medical University, Dalian 116085, Liaoning Province, China; 3Department of Conservative & Endodontic Dentistry, Affiliated Stomatological Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China
  • Revised:2014-01-11 Online:2014-03-12 Published:2014-03-12
  • Contact: Ma Guo-wu, M.D., Professor, Department of Oral and Maxillofacial Surgery, Stomatology College of Dalian Medical University, Dalian 116085, Liaoning Province, China
  • About author:Yang Ying, Master, Physician, Department of Oral Implantology, Affiliated Stomatological Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China

PDF

452

被引次数

4

摘要：

背景：目前，自体骨髓浓缩物主要应用于骨科、重症肢体缺血患者，其在口腔颌面外科尤其是种植外科应用较少，在种植体周围促进新骨再生的效果也有待研究。

目的：评价自体骨髓浓缩物在种植体周围骨缺损修复中的作用。
方法：于实验犬髂后上棘处抽取骨髓5 mL，对浓缩前后的骨髓进行细胞计数。在实验犬的双侧下颌前磨牙区植入种植体，并于近中制作4 mm×4 mm×4 mm的骨缺损，随机植入骨髓浓缩物混合明胶海绵、自体骨、明胶海绵。术后4，12周对骨缺损标本进行组织学观察分析，并计算新骨生成率和新骨密度。

结果与结论：骨髓细胞平均浓缩倍数为(2.78±0.22)倍。浓缩骨髓中，可观察到更多和更大的克隆单位。组织学分析发现，4周时骨髓浓缩物明胶海绵组的新骨生成率和密度均明显高于自体骨组和明胶海绵组(P < 0.05)，12周时骨髓浓缩物明胶海绵组新骨生成率明显低于自体骨组，但高于明胶海绵组(P < 0.05)，3组新骨密度差异无显著性意义(P > 0.05)。结果显示自体骨髓浓缩物能够显著提高种植体周围骨缺损内早期新生骨的质量。



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

关键词: 组织构建, 骨组织构建, 种植体, 骨缺损, 骨髓浓缩物, 骨再生

Abstract:

 BACKGROUND: Autologous bone marrow aspirate concentrate is often applied in patients from Department of Orthopedics and those with severe limb ischemia, but rarely applied in Department of Oral and Maxillofacial Surgery, especially in Department of Oral Implantology. The effect of autologous bone marrow aspirate concentrate on promoting peri-implant bone regeneration deserves further studies.

OBJECTIVE: To evaluate the effect of bone marrow aspirate concentrate in the repair of peri-implant bone defect.
METHODS: Bone marrow 5 mL was extracted from posterior superior iliac spine of experimental dogs and bone marrow cells were counted before and after concentration. Bone defect (4 mm × 4 mm × 4 mm) was prepared in the middle of bilateral mandibular premolar, which was randomly implanted with gelatin sponge plus bone marrow aspirate concentrate, autologous bone and gelatin sponge. At 4 and 12 weeks after surgery, bone defect specimens were histologically observed. The new bone formation rate and new bone mineral density were calculated.
RESULTS AND CONCLUSION: After centrifugation, the concentrations of nucleated cells in bone marrow aspirate concentrate were increased by (2.78±0.22) times. More colony-forming units were found after cell culture. Histological analysis showed that, significantly higher new bone formation rate and new bone mineral density occurred in gelatin sponge plus bone marrow aspirate concentrate group, compared with autologous bone group and gelatin sponge group at 4 weeks (P < 0.05). The new bone formation rate in gelatin sponge plus bone marrow aspirate concentrate group was significantly lower than that of autologous bone group, and higher than that of gelatin sponge group at 12 weeks (P < 0.05). However, the difference of new bone mineral density in the three groups was not significant (P > 0.05). Autologous bone marrow aspirate concentrate can significantly improve new bone mineral density and quantity in the pre-implant bone defect.


中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

Key words: dental implants, bone marrow, bone regeneration, gelatin sponge, absorbable

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